张鸿卿, 柳惠图, 邓藻锡, 潘维林, 胡云英, 薛绍白. 平阳霉素(博菜霉素A5)对同步化的CHO细胞的敏感周期时相的研究J. 药学学报, 1982, 17(10): 755-760.
引用本文: 张鸿卿, 柳惠图, 邓藻锡, 潘维林, 胡云英, 薛绍白. 平阳霉素(博菜霉素A5)对同步化的CHO细胞的敏感周期时相的研究J. 药学学报, 1982, 17(10): 755-760.
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ZHANG Hong-qing, LIU Hui-tu, DENG Zao-xi, PAN Wei-lin, HU Yun-ying , XUE Shao-bai, . CELL CYCLE PHASE SENSITIVITY OF CHO CELL TO PINGYANGMYCINJ. Acta Pharmaceutica Sinica, 1982, 17(10): 755-760.
Citation: ZHANG Hong-qing, LIU Hui-tu, DENG Zao-xi, PAN Wei-lin, HU Yun-ying , XUE Shao-bai, . CELL CYCLE PHASE SENSITIVITY OF CHO CELL TO PINGYANGMYCINJ. Acta Pharmaceutica Sinica, 1982, 17(10): 755-760.
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平阳霉素(博菜霉素A5)对同步化的CHO细胞的敏感周期时相的研究

CELL CYCLE PHASE SENSITIVITY OF CHO CELL TO PINGYANGMYCIN

    摘要
  • 摘要: 平阳霉素(博莱霉素A5)作用于同步化的各个周期时相的CHO细胞。结果用集落形成率来表示。研究表明平阳霉素对M期细胞最为敏感,其敏感顺序为M>G2>S>G1。实验使用的细胞同步化方法是用有丝分裂选择法收集M期细胞;用2 mMTdR双阻断法获得S期同步化细胞,再由M期和S期细胞分别向后推移1.5～2小时和7小时以获得G1期和G2期的同步细胞,并附以有丝分裂指数(MI)和标记指数(LI)检测各周期时相细胞的同步化程度。

     

    Abstract: The effect of Pingyangmycin on relative survival of Chinese hamster ovary cells growing in vitro at various stages in the cell cycle was studied. It was shown that under the dosage used Pingyangmyein is most effective in killing the cells in mitosis and G2 phase. According to their sensitivities to the drug the sensitivity of the cells ranked as follows: M>G2>S>G1.

     

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