Abstract: AimTo study on synthesis and antibacterial activity evaluation of polyheterocycles. MethodsThe condensation of 4-amino-3-pyridin-3-yl-4H-［1,2,4］ triazole-5-thiol with 2-chloromethyl-5-substituted phenyl-［1,3,4］oxadiazoles gave the corresponding title heterocycle amines, and the in vitro antibacterial activity was primarily evaluated by the method of cup-plate diffusion solution. Results Twelve novel compounds were synthesized, and their structures were confirmed by IR, ¹H NMR, MS and element analysis. Biological screening results demonstrated that most of the compounds prepared showed good antibacterial activity. ConclusionOxadiazoles incorporting pyridyl triazole ring may be a pharmacophor structure in the molecule for developing antibacterial candidate drugs.