陈庆文, 单宏丽, 孙宏丽, 王赫, 杨宝峰. 环维黄杨星D对分离大鼠心室肌细胞内Ca2+和L型钙电流的影响J. 药学学报, 2004, 39(7): 500-503.
引用本文: 陈庆文, 单宏丽, 孙宏丽, 王赫, 杨宝峰. 环维黄杨星D对分离大鼠心室肌细胞内Ca2+和L型钙电流的影响J. 药学学报, 2004, 39(7): 500-503.
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CHEN Qing-wen, SHAN Hong-li, SUN Hong-li, WANG He, YANG Bao-feng. Effects of cyclovirobuxine D on intracellular Ca2+ and L-type Ca2+ current in rat ventricular cardiomyocytesJ. Acta Pharmaceutica Sinica, 2004, 39(7): 500-503.
Citation: CHEN Qing-wen, SHAN Hong-li, SUN Hong-li, WANG He, YANG Bao-feng. Effects of cyclovirobuxine D on intracellular Ca2+ and L-type Ca2+ current in rat ventricular cardiomyocytesJ. Acta Pharmaceutica Sinica, 2004, 39(7): 500-503.
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环维黄杨星D对分离大鼠心室肌细胞内Ca2+和L型钙电流的影响

Effects of cyclovirobuxine D on intracellular Ca2+ and L-type Ca2+ current in rat ventricular cardiomyocytes

    摘要
  • 摘要: 目的研究环维黄杨星D（CD）对大鼠心室肌细胞内Ca2+动员和L型钙电流(ICa-L/sub>)的影响。方法采用全细胞膜片钳和激光扫描共聚焦显微术研究CD对心肌细胞ICa-L/sub>以及氯化钾、咖啡因诱发心肌细胞内Ca2+动员的影响。结果CD浓度依赖性抑制ICa-L/sub>。指令电压为10 mV时，1和10 μmol·L-1 CD分别使ICa-L/sub>电流密度从（-9.9±1.8）pA/pF降至（-6.4±1.4）pA/pF和（-4.2±0.6）pA/pF。共聚焦实验显示1和10 μmol·L-1 CD不影响静息心肌细胞［Ca2+］i?/sub>，对氯化钾诱发［Ca2+］i?/sub>升高水平无明显抑制作用；咖啡因引起的细胞内Ca2+动员可被CD进一步增强。结论CD浓度依赖性抑制大鼠心室肌细胞ICa-L/sub>，并有促进咖啡因诱发心肌细胞内Ca2+释放的作用。

     

    Abstract: AimTo determine the effects of cyclovirobuxine D (CD) on intracellular Ca2+ mobilization and L-type Ca2+ current (ICa-L/sub>) in isolated rat cardiomyocytes. MethodsThe effects of CD on the amplitude of ICa-L/sub> and intracellular Ca2+mobilization induced by KCl and caffeine were studied with the method of patch-clamp technique and laser scanning confocal microscopy in rat ventricular myocytes. ResultsCD decreased the amplitude of ICa-L/sub> in a concentration-dependent manner. At 10 mV, 1 and 10 μmol·L-1 CD decreased ICa-L/sub> density from (-9.9±1.8) pA/pF to（-6.4±1.4）and（-4.2±0.6）pA/pF, respectively. Confocal experiments showed that intracellular fluorescent intensity(FI)value of ［Ca2+］i?/sub> in control resting level was not changed by 1 and 10 μmol·L-1 CD. ［Ca2+］i?/sub> increase in response to KCl could not be reduced by CD. The rise of ［Ca2+］i?/sub> in response to caffeine was further enhanced by pretreatment with CD. ConclusionCD decreased ICa,L in a concentration-dependent manner and increased ［Ca2+］i?/sub> release induced by caffeine in rat ventricular cardiomyocytes.

     

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