反相离子对色谱法测定附子中生物碱成分

Determination of alkaloids in Radix Aconiti Lateralis Preparata by RP-ion-pair HPLC

摘要: 目的测定附子中乌头碱、新乌头碱、次乌头碱、北乌碱、苯甲酰乌头原碱和苯甲酰新乌头原碱等6种生物碱的含量。方法用反相离子对HPLC法，使用AichromBond-1 C₁₈柱(250 mm×4.6 mm ID)；流动相：乙腈-5 mmol·L^-1 NaH₂PO₄溶液，磷酸调至pH 4.5(50∶50)，内含7 mmol·L^-1 十二烷基硫酸钠(SDS)；检测波长：235 nm；流速：1.0 mL·min^-1；柱温：35 ℃。结果以上6种生物碱可以完全分离，准确测定。结论该方法准确度高，可应用于附子中生物碱的含量测定。

Abstract: AimTo separate and quantitatively determine six alkaloids: aconitine, mesaconitine, hypaconitine, beiwutine, benzoylaconine and benzoylmesaconine in the Chinese traditional medicine Radix Aconiti Lateralis Preparata (Fuzi). MethodsA RP-ion-pair HPLC method was established. An AichromBond-1 C₁₈ column was used at a column-temperature of 35 ℃. The mobile phase was CH₃CN-5 mmol·L^-1 NaH₂PO₄ (50∶50) containing 7 mmol·L^-1 SDS at a flow-rate of 1.0 mL·min^-1. The detector was set at UV 235 nm. ResultsThese six alkaloids can be completely separated and determined quantitatively. ConclusionThis method is accurate and suitable for the determination of six alkaloids in Fuzi.