Abstract: AimTo establish a fingerprint analysis method of Fritillaria hupehensis. MethodsThe fingerprint was performed by HPLC-ELSD. Hypersil ODS column was used; the mobile phase was composed of methanol (with 0.05% triethylamine) and water with gradient elution; flow rate was 1.0 mL·min^-1; recording time was 60 min; drift tube temperature was 75 ℃; gas flow rate was 1.9 L·min^-1. ResultsHPLC fingerprint of Fritillaria hupehensis was obtained. ConclusionA reliable method was provided for controlling the quality of Fritillaria hupehensis.