广藿香ITS基因型与地理分布的相关性分析

Correlation between ITS genotype and geographical distribution of Pogostemon cablin

摘要: 探讨广藿香Pogostemon cablin (Blanco) Benth.的地理分布与ITS基因型的关系，为广藿香的道地性分析和种质评价提供分子依据。采用PCR直接测序技术对不同产地的广藿香ITS₁和ITS₂基因进行测序分析。结果显示，不同产地广藿香ITS₁基因和ITS₂基因核苷酸序列长度分别为424 bp和380 bp，其中前者存在19个变异位点，后者存在5个变异位点，说明广藿香的地理分布和其ITS₁，ITS₂基因型具有良好的相关性，因而ITS₁和ITS₂测序分析可为广藿香的地理分布与基因型的相关性及其种质评价提供有用的分子信息。

Abstract: To investigate the correlation between genotype and distribution of Pogostemon cablin by sequencing ITS₁ and ITS₂ genes, and provide molecular information for its germplasm evaluation, ITS₁ and ITS₂ genes of Pogostemon cablin from different localities were identified by PCR direct sequencing. The sequences of ITS₁ and ITS₂ genes were 424 bp and 380 bp in length, respectively. And nineteen base substitutions were observed in ITS₁ gene, and five in ITS₂ gene. The results showed a good correlation between genotype and distribution of Pogostemon cablin, and ITS gene sequencing could provide useful molecular information for germplasm evaluation of the plant species verification.