Abstract: AimTo study the chromatographic behavior of cetirizine dihydrochloride on the proteinate- and amylose- based chiral stationary phases so as to optimizate the chromatographic condition of its enantiomers separation. MethodsWhen using amylose-based, α₁-acid glycoprotein and ovomucoid protein chiral stationary phase, the mobile phase was hexane-isopropyl alcohol-alcohol-trifluoroacetic acid (430∶45∶25∶1), acetonitrile-10 mmol·L^-1 phosphate buffer solution (adjusted to pH 7.0 with sodium hydroxide) (4∶96) and acetonitrile -20 mmol·L^-1 KH₂PO₄ solution (adjusted to pH 7.0 with triethylamine) (12.7∶87.3), respectively. The temperature of proteinate column was 25 ℃. The detective wavelength was 230 nm. Results The two enantiomers could be separated on the two kinds of chiral stationary phases without derivatization and the resolution was above 2.0. The methods developed on the two kinds of chiral stationary phases are accurate, sensitive and specific. ConclusionBoth the proteinate- and amylose-based chiral stationary phases can be used to separate the enantiomers of cetirizine.