Abstract: AIMTo study the chemical composition in the fruits of Liquidambar formana Hance. METHODS Various column chromatographic techniques were used to separate and purify the constituents. Their physical and chemical properities and spectral data were measured for structural elucidation. RESULTSEleven compounds were isolated and identified as β-sitosterol (1), 3-oxo-11α,12α-epoxyoleanan-28,13β-olide (2), 3-oxo-12α-hydroxy-oleanan-28,13β-olide (3), 3α-acetyloxy-25-hydroxyolean-12-en-28-oic acid (4), oleanolic acid (5), ursolic acid (6), daucosterol (7), betulonic acid (8), gallic acid (9), nonacosane (10) and n-triacontanoic acid (11). CONCLUSION Among the isolated constituents, compound 4 is new compound, compound 3 is firstly isolated from the natural product and compound 5, 6, 7, 9, 10 and 11 are isolated from LuLuTong for the first time.