超临界流体萃取-高效液相色谱法测定何首乌中磷脂成分

DETERMINATION OF PHOSPHOLIPID IN POLYGONUM MULTIFLORUM THUNB. BY SUPERCRITICAL FLUID EXTRACTION—HPLC

摘要: 目的：建立超临界流体萃取(SFE)中药何首乌中的磷脂类成分的方法。方法：采用系统观察法考察了SFE的提取工艺,并用反相高效液相色谱法进行分离测定。结果：在SFE中，通过对萃取条件的考察，确定萃取压力为31.5 MPa,温度50℃,改性剂加入量0.6 mL.g^-1,静态萃取时间5 min及动态萃取体积7 mL。在HPLC中,以Waters Symmetry C₁₈为固定相,甲醇—1.0%磷酸溶液(90∶10)为流动相,检测波长206 nm。结论：SFE-HPLC法测定何首乌中的磷脂类成分，为何首乌及其制剂的质量控制提供依据。

Abstract: AIM： To develop a supercritical fluid extraction (SFE) method for the phospholipids of Polygonum multiflorum Thunb. METHODS: SFE was selected by systemic research method, an HPLC method was developed for the determination of phosphatidylethanolamine and phosphatidylcholine. RESULTS: SFE conditions: pressure 31.5 MPa, temperature 50℃, 0.3 mL.g^-1 modifier. The samples were extracted statically for 5 min, and subsequently extracted dynamically with 7 mL extractant. HPLC conditions: The HPLC column was packed with C₁₈ (5 μm). The mobile phase was methanol—1% phosphoric acid (90∶10). The detection wavelength was 206 nm. CONCLUSION: The method is accurate, convenient and rapid, and can be a measure of quality control for Polygonum multiflorum Thunb. and its preparations.