叶酸的光化学行为及其应用

PHOTOCHEMICAL BEHAVIOUR OF FOLIC ACID AND ITS APPLICATION

摘要: 目的：研究叶酸的光化学行为并提出测定叶酸的光化学荧光分析方法。方法：叶酸在六次甲基四胺-盐酸介质中,经365 nm紫外光照射后，发生光化学反应，产物的荧光强度(λ_ex 280 nm,λ_em 443 nm)较叶酸本身的增大了25倍。根据光化学反应产物的荧光强度测定叶酸的含量。结果：叶酸的浓度在1.0×10^-5～1.0×10^-7 mol.L^-1范围内，荧光强度与浓度呈良好的线性关系，检出限为1.5×10-9 mol.L^-1，相对标准偏差为1.6%。结论：本方法适用于片剂中叶酸的测定。

Abstract: AIM: To study the photochemical behaviour of folic acid and establish a photochemical fluorimetric method for folic acid determination. METHODS: In (CH₂)₆N₄-HCl medium, a photochemical reaction takes place on irradiation of the folic acid with 365 nm ultravident light. The photochemical product showed an intense fluorescence intensity (λex 280 nm, λem 443 nm), which was 25 times higher than that of the original folic acid. On this basis, the determination of folic acid was carried out. RESULTS: The linear relationship between the fluorescence intensity and concentration of folic acid is over the range of 1.0×10^-7～1.0×10^-5 mol.L^-1, the detection limit is 1.5 nmol.L^-1 and relative standard deviation is 1.6%. The recoveries are in the range of (99.5～106.5)%. CONCLUSION: It can be applied to the determination of folic acid in tablets.