非水毛细管电泳法同时测定麦冬中薯蓣皂苷元和鲁斯可皂苷元的含量

etermination of diosgenin and ruscogenin in Radix Ophiopogonis by nonaqueous capillary electrophoresis

摘要:
采用非水介质毛细管电泳法同时测定了麦冬中薯蓣皂苷元和鲁斯可皂苷元的含量。以70%甲醇为非水介质, 20 mmol·L⁻¹硼砂-HCl为缓冲溶液 (pH 7.61), 运行为25 kV高压, +0.70 V工作电极电位的条件下, 实现了被测组分的有效分离。测得麦冬中薯蓣皂苷元的含量为0.018 mg·g⁻¹, 鲁斯可皂苷元的含量为0.008 mg·g⁻¹, 薯蓣皂苷元和鲁斯可皂苷元的平均回收率分别为102%和99.2%。本法为麦冬中薯蓣皂苷元和鲁斯可皂苷元的质量控制提供了新方法。

Abstract:
Nonaqueous capillary electrophoresis is used for the determination of the contents of diosgenin and ruscogenin in Radix Ophiopogonis. The operating buffer was composed of 20 mmol·L⁻¹ Na₂B₄O₇-HCl (pH 7.61) in 70% methanol. The applied voltage was 25 kV and detection potential was at +0.70 V. With these conditions, the components were successfully separated. The content of diosgenin in Radix Ophiopogonis was 0.018 mg·g⁻¹ and ruscogenin was 0.008 mg·g⁻¹. The average recoveries of diosgenin and ruscogenin were 102% and 99.2%, respectively. A new method of the quality control of diosgenin and ruscogenin in Radix Ophiopogonis is provided.