Abstract: AimTo investigate the chemical constituents of Dendrobium fimbriatum Hook. Methods Various chromatographic techniques were employed for isolation and purification of the constituents. The structures were elucidated by IR, MS, ¹HNMR, ¹³CNMR and 2D-NMR. ResultsEight compounds were obtained and identified by spectral analysis as fimbriatone, confusarin, crepidatin, physcion, rhein, ayapin, scopolin methyl ether and n-octacostyl ferulate. ConclusionFimbriatone is a new compound, physcion and rhein are firstly isolated from Genus Dendrobium. The others are found from this species for the first time. Fimbriatone showed potential inhibitory effects on BGC cell line.