A rapid ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometric (UPLC-ESI-MS/MS) method is developed for the qualitative identification of constituents in the flower buds of seven Lonicera species.  The optimal condition of separation and detection were achieved on an AcQuity UPLC^TM BEH C₁₈ column with a gradient elution with acetonitrile and 0.1% acetic acid within 17 min.  Among the 33 constituents detected, 6 caffeoylquinic acids (including caffeic acid), 8 flavonoids and 8 iridoid glycosides were characterized based on their fragmentation patterns in collision-induced dissociation (CID) experiments and/or by comparison with standard compounds.  In addition, to statistically establish the correlation and    discrimination of the Lonicera species, principle component analysis (PCA) was applied in this study.  Lonicera samples were divided into well-defined groups directly related to their species based on PCA in terms of the log transformed relative contents of the major caffeoylquinic acids (including caffeic acid) as the variables.  All of results indicated that the method presented here is able to classify the sample species and to reveal characteristic details of the chemical constituents of different Lonicera species.