蝎毒镇痛活性肽在毕赤酵母中表达条件的优化

Optimization on the production of analgesic peptide from Buthus martensii Karsch in Pichia pastoris

摘要: 采用单因子和正交试验对毕赤酵母重组菌产生蝎毒镇痛活性肽BmK AngM1的发酵过程进行优化, 确定了最佳发酵条件: 1.2%甲醇, 0.6%酪蛋白水解物, 初始pH 6.0, 3×接种量。在此发酵条件下, BmK AngM1在毕赤酵母重组菌中的表达量可超过500 mg·L^-¹, 比对照提高了两倍以上。本研究为大规模发酵制备BmK AngM1奠定了基础, 更好地满足了BmK AngM1的理论研究及新药开发的需要。

Abstract: The technology of liquid fermentation for producing the recombinant analgesic peptide BmK AngM1 from Buthus martensii Karsch in Pichia pastoris was studied by single-factor and orthogonal test. The results showed that the optimal culture conditions were as follows: 1.2% methanol, 0.6% casamino acids, initial pH 6.0, and three times of basal inoculation volume. Under the above culture conditions, the expression level of recombinant BmK AngM1 in Pichia pastoris was above 500 mg·L^-¹, which was more than three times of the control. The study has laid a foundation for the large-scale preparation of BmK AngM1 to meet the needs of theoretical research of BmK AngM1 and development of new medicines.