丁基苯酞对原代培养胎大鼠皮层神经细胞外液NO及胞浆内cGMP水平的影响

EFFECTS OF D-3-N-BUTYLPHTHALIDE AND L-3-N-BUTYLPHTHALIDE ON EXTRACELLULAR NO LEVELAND INTRACELLULAR cGMP LEVEL IN PRIMARY CULTURED RAT CORTICAL NEURONS

摘要: 用分光光度法和放射免疫分析法分别检测NO及cGMP水平,同时观察l-丁基苯酞(l-NBP)和d-丁基苯酞(d-NBP)对原代培养的胎大鼠皮层神经细胞外液NO及胞浆内cGMP水平的影响。结果表明,d-NBP(0.1～100μmol·L^-1)对低糖低氧条件下或含N-甲基-D-门冬氨酸(NMDA)或含KCl的培养基中皮层神经细胞外液NO及细胞内cGMP水平明显升高,而l-NBP(0.1～100μmol·L^-1)则能明显降低NO和cGMP水平。提示:d-NBP和l-NBP对低糖低氧,NMDA或KCl诱导的NO释放和cGMP生成有相反的作用。

Abstract: The effects of l-3-n-butylphthalide(l-NBP) and d-3-n-butylphthalide(d-NBP) on extracellular nitric oxide(NO) levels and intracellular cyclic GMP(cGMP) levels were studied in primary cultured rat cortical neuronal cells. Nitric oxide and cGMP levels were measured by using spectrometry and radioimmunological analysis(RIA), respectively. The results showed that d-NBP(0.1～100μmol·L^-1) markedly increased extracellular NO levels and intracellular cGMP levels in primary cultured neurons that were exposed for 10 h to hypoxic/hypoglycemic, n-methyl-D-aspartate (NMDA), or KCl media. On the contrary, l-NBP(0.1～100μmol·L^-1) significantly decreased extracellular NO levels and intracellular cGMP levels. It is suggested that there is a contrary effect of d-NBP and l-NBP on NO release and cGMP production induced by hypoxia/hypoglycemia, NMDA, or KCl.