Abstract: AIM　To establish a TLC fluorescent scanning method for separation and determination of the six stilbenes: isorhapotigenin-3-O-β-D-glucopyranoside (I), gnetifolin C (II), ε-viniferin (III), resveratol (IV), isorhapotigenin (V) and pinosylvine (VI) in Gnetum parvifolium. METHODS　The separation of stilbenes II～VI was accomplished on the silica gel plate by twice development using the mobile phase toluene-glacial acetic acid-methanol (3∶1∶0.5). The separation of I was achieved by using the mobile phase toluene-ethyl acetate-glacial acetic acid-methanol(1∶2∶0.5∶0.2). After separation of the stilbenes, the plate was dipped into a mixture of paraffin oil-hexane (1∶1) to protect the fluorescence. The contents of six stilbenes were determined by HPTLC fluorescent scanning with λ_ex=313 nm and λ_em=400 nm (No.2 filter). RESULTS The calibration curves showed good linearity in the range of 17.4～260.7 ng. The recoveries were 96.4%～104.8% with RSD% 2.3%～5.1%. The method has been applied to the analysis of various samples. CONCLUSION　It offered a simple, sensitive, and accurate method for research and utilization resources of stilbenes.