11种獐牙菜及近缘植物中有效成分的高效液相色谱测定

DETERMINATION OF EFFECTIVE CONSTITUENTS IN 11 SWERTIA AND RELATED PLANTS HPLC

摘要: 建立了獐牙菜中3种苦味甙,即獐牙菜苦甙(swertiamarin)、獐牙菜甙(sweroside)、龙胆苦甙(gentiopicroside)及一种　酮甙──獐牙菜　酮甙(swertianolin)的高效液相色谱定量分析方法(μ-BondpakC₁₈柱,水—甲醇—异丙醇—四氢呋喃=65:30:5:1作流动相);对11种獐牙菜及近缘植物椭圆叶花锚、坚龙胆进行了含量测定,并对他们之间的异同进行了比较。

Abstract: This paper deals with quantitative determination of three iridoids──swertiama-tin,gentiopicroside ,sweroside and a xanthone──swertianolin in eleven Swertia species and related plants by means of HPLC.μ-Bondapak C₁₈ was used as the stationary phase ,and water─methanol─isopropanol─tetrahydrofuran(65 :30:5:1) as the mobile phase. Analytical results were given.The similarities and diversities of these plants were compared and discussed.