张烜, 黄力新, 聂松青, 齐宪荣, 张强. 包裹在脂质体内部胰岛素的二级结构J. 药学学报, 2003, 38(11): 863-866.
引用本文: 张烜, 黄力新, 聂松青, 齐宪荣, 张强. 包裹在脂质体内部胰岛素的二级结构J. 药学学报, 2003, 38(11): 863-866.
shu
ZHANG Xuan, HUANG Li-xin NIE Song-qing, QI Xian-rongZHANG Qiang, . Secondary structure of insulin encapsulated within liposomesJ. Acta Pharmaceutica Sinica, 2003, 38(11): 863-866.
Citation: ZHANG Xuan, HUANG Li-xin NIE Song-qing, QI Xian-rongZHANG Qiang, . Secondary structure of insulin encapsulated within liposomesJ. Acta Pharmaceutica Sinica, 2003, 38(11): 863-866.
shu

包裹在脂质体内部胰岛素的二级结构

Secondary structure of insulin encapsulated within liposomes

    摘要
  • 摘要: 目的以单纯胰岛素为对照，采用傅立叶红外光谱（Fourier transform infrared， FTIR）研究包裹在脂质体内部胰岛素二级结构的变化。方法分别对单纯胰岛素、胰岛素与空白脂质体混合物（样品I）及包裹胰岛素的脂质体（II）样品进行测定。结果样品I和样品II中胰岛素的FTIR谱图形状与单纯胰岛素相比基本一致，仅α-螺旋百分比略有下降（由36.09%分别下降到31.68%和31.45%），β-折叠百分比略有增加（由47.83%分别增加到53.29%和51.36%）。样品I和样品II中胰岛素的二级结构无明显差别（α-螺旋百分比为31.68%和31.45%，β-折叠百分比为53.29%和51.36%）。结论包裹在脂质体内部胰岛素的二级结构与单纯胰岛素相比无明显变化。

     

    Abstract: AimTo determine the secondary structure of insulin encapsulated within liposomes. MethodsThe secondary structure of insulin, mixture of insulin with liposomes （I） and insulin liposomes （II） were determined by Fourier transform infrared (FTIR) spectroscopy. ResultsThe figures of secondary structure of insulin, sample I and II were similar. The results showed that the amount of α-helix and β-sheet of insulin，sample I and II had little difference, from 36.09% (insulin) to 31.68% (sample I) and 31.45% (sample II), and from 47.83% (insulin) to 53.29% (I) and 51.36% (II), respectively. The results showed that the insulin of sample I and II did not insert in lipsomes membrane, only adsorbed or extendedon the surface of the lipsomes. ConclusionThe secondary structure of insulin encapsulated within liposomes has not been destroyed and still remain the original state.

     

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