药典内5种淫羊藿中黄酮类成分的反相高效液相色谱分析
DETERMINATION OF 9 FLAVONOIDS IN 5 SPECIES OF EPIMEDIUM RECORDED IN CHINESE PHARMACOPOEIA BY HPLC
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摘要: 报道了药典规定的5种淫羊藿──淫羊藿、箭叶淫羊藿、巫山淫羊藿、柔毛淫羊藿和朝鲜淫羊藿中9种黄酮──淫羊藿甙(icariin)、宝藿甙-Ⅰ(baohuosideI)、宝藿甙-Ⅱ(baohuosideⅡ)、淫羊藿甙A(epimedosideA)、箭藿甙B(sagittatosideB)、朝藿定B(epimedinB)、朝藿定C(epimedinC)、大花淫羊藿甙C(ikarisosideC)和大花淫羊藿甙F(ikarisosideF)的反相高效液相色谱测定。色谱柱为DISK-Cphenlyl,流动相为乙腈-乙酸液(水:36%乙酸=100:4),梯度洗脱,检测波长为272nm。Abstract: An accurate RP-HPLC method for the determination of icariin,baohuoside baohuoside Ⅱ,epimedoside A,sagittatoside B,epimedin B,epimedmc,ikarisoside C and ikarisosideF in the leaves of Epimedium brevicornum Maxim.,E. sagittatum(Sieb.et Zucc.)Maxim.,E.wushanense T.S.Ying,E.pubescens Maxim. and E. koreanum Nakai has been developed. The analytieal column wasDISK-Cphenly. The mobile phase was acetonitrile-acetic acid solution. Gradient elution was employed.The detection wavelength was 272 nm.
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