Abstract: Eight constituents were isolated from the dried herb of Artemisia rupestris L. Seven of them were identified as aciphyllic acid, cis and trans-spiroketalenoetherpolyne, gardenin D, β-sitosterol, β-sitosterol-3-O-β-glucoside and palmitic acid, respectively. The other one named rupestric acid (Ⅳ)has not been reported previously in the literature. The structure of rupestric acid has been elucidated by spectroscopic data (UV, IR, MS, NMR) and chemical transformation.