杨云鹏, 岳德超, 霍泽民. 薄盖灵芝菌深层发酵培养的研究J. 药学学报, 1979, 14(7): 434-438.
引用本文: 杨云鹏, 岳德超, 霍泽民. 薄盖灵芝菌深层发酵培养的研究J. 药学学报, 1979, 14(7): 434-438.
Yang Yunpeng, Yue Dechao , Huo Zemin, . STUDIES ON THE SUBMERGED CULTIVATION OF GANODERMA CAPENSEJ. Acta Pharmaceutica Sinica, 1979, 14(7): 434-438.
Citation: Yang Yunpeng, Yue Dechao , Huo Zemin, . STUDIES ON THE SUBMERGED CULTIVATION OF GANODERMA CAPENSEJ. Acta Pharmaceutica Sinica, 1979, 14(7): 434-438.

薄盖灵芝菌深层发酵培养的研究

STUDIES ON THE SUBMERGED CULTIVATION OF GANODERMA CAPENSE

  • 摘要: 为了解决临床药源,对薄盖灵芝(Ganoderma capense (Lloyd)Teng)Gcl菌种进行了深层发酵培养的研究。所得结果是:选用4号发酵培养基(由蔗糖4%,豆饼粉2%,KH2PO40.15%,CaCO3 0.05%,MgSO4·7H2O 0.075%及(NH4)2SO40.05%组成),在24~26℃下,培养120~144小时,发酵液为黄棕色,布满菌丝,静止后澄清透明,pH值约为5左右,残糖量降至0.3左右,具有清香味,这时即可终止发酵。发酵物经提取后制成针剂及片剂,供临床应用。

     

    Abstract: In order to produce large amount of mycelia of Ganoderma capense (Lloyd) Teng by submerged culture technique, a study has been made on the fermentation conditions of the fungus. The mycelium of this fungus under submerged condition is colorless, filamentous, septate and branched.The following optimal conditions were verified, the medium composition (No.4): 4.0% sucrose, 2.0% soybean cake meal, 0.15% KH2PO4, 0.075% MgSO4·7H2O and 0.05% (NH4)2 SO4, with natural pH, optimal amount of inoculum being 10%, rate of aeration 0.3~0.5 (v/v/rain.). The inoculated cultures were incubated at 24~26℃ for 5~6 days.Fermentation is usually stopped as the pH dropped to about 5.0 and residual sugar to aboul 0.3%.

     

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