Abstract: The effects of neferineNef), taurine(Tau) and Nef+Tau on platelet aggrega-tion and generation of thrombosis were investigated in rats, Usomg turbidimetry Nef and Tau were found to inhibit platelet aggregation induced by adenosine diphosphate (ADP),collagen or thrombin.Both drugs reducde the wet weight of experimeental thrombosis.Bit the drugs showed no obvious dffect on fibrinogen content and euglobulin lysis time, Thromboxane A₂(TXA₂ )production induced by ADP and prostacyclin(PGI₂ ) were studied by enzyme immunoassay in rats. TXA₂ generation in platelet rich plasma from rats treated with Nef and Nef+Tau obviously decreased. While the plasma PGI₂ was not affected, Nef+Tau iv in 30 min significantbly decreased the level of total cholesterin in serum.The results suggest that Nef has inhibitory effect on platelet aggregation and thrombosis formation.The combination of both drugs was found to be more potent than either one alone,The mechanism may be related to its reduction of TXA₂ formation.