以流感病毒RNA聚合酶为靶点的抗病毒药物筛选和药效学评价方法的建立

Establishment of drug screening assay and pharmacodynamic evaluation method targeting influenza RNA polymerase

摘要:
流感病毒RNA聚合酶对流感病毒基因组的复制和表达至关重要。编码聚合酶各亚基的基因序列高度保守的特点使其成为极具发展潜力的抗流感药物靶标。本研究通过构建流感病毒RNA聚合酶活性依赖的荧光素酶报告系统, 在细胞水平上建立了以流感病毒RNA聚合酶为靶点的抗流感药物筛选模型。特异性评价和统计学分析表明, 该筛选方法灵敏可靠、重复性好, 可以用于针对流感病毒RNA聚合酶的抗流感药物筛选。

Abstract:
Influenza virus RNA-dependent RNA polymerase (RdRP) is essential for replication and expression of influenza virus genome. Viral genomic sequences encoding RdRP are highly conservative, thus making it a potential anti-influenza drug target. A cell-based influenza RdRP inhibitor screening assay was established by a luciferase reporter system to analyze the activity of RdRP. Specificity study and statistic analysis showed that the screening assay is sensitive and reproducible.