前体及真菌诱导子对银杏悬浮培养细胞产生银杏内酯B的影响

戴均贵; 朱蔚华; 吴蕴祺; 胡秋; 张大勇

前体及真菌诱导子对银杏悬浮培养细胞产生银杏内酯B的影响

EFFECTS OF PRECURSORS AND FUNGAL ELICITORS ON GKB PRODUCTION IN SUSPENSION CULTURED CELLS OF GINKGO BILOBA L.

摘要

摘要: 目的：探索前体及真菌诱导子对银杏悬浮培养细胞产生银杏内酯B的影响。方法：通过向培养基中补加前体及真菌诱导子，考察它们对悬浮培养细胞生物量及银杏内酯B产量的影响。结果：于培养基中添加100 mg.L^-1异戊二烯及低浓度(10 mg.L^-1，50 mg.L^-1)的　牛儿醇能提高GKB的产量,分别比对照增加了69%，13.8%和11.4%。从10种真菌诱导子中筛选出效果较好的日本根霉诱导子，当添加浓度为5 mg GE.L^-1，诱导培养3 d时，悬浮细胞的GKB产量比对照增加约1倍。结论：在银杏悬浮细胞培养过程中，添加前体物质及应用真菌诱导子是提高银杏内酯B的有效手段之一。

Abstract: AIM: To investigate the effects of precursors and fungal elicitors on the GKB production in suspension cultured cells of Ginkgo biloba L. METHODS: Precursors and fungal elicitors were added to the media. Their effects on the biomass and GKB yields of the suspension of cultured cells were studied. RESULTS: The total GKB yields were enhanced 69%, 13.8% and 11.4% compared with the control by adding 100 mg.L^-1 of isoprene and low concentrations (10 and 50 mg.L^-1) of geraniol in the medium, respectively. Of the 10 investigated fungal elicitors, mycelium extract of Rhizopus japonicus was found to be the best, and at the concentration of 5 mg GE.L^-1, the GKB yield of suspension cells was doubled after the cells were induced for 3 days. CONCLUSION: Adding precursors in the media and applying fungal elicitors were both effective approaches to enhance GKB yields in the suspension culture cell of Ginkgo biloba L.

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