戊型肝炎病毒(HEV)蛋白抗原肽的化学合成及在血清学诊断中的应用

CHEMICAL SYNTHESIS OF ANTIGENIC PEPTIDES FROM PROTEIN OF HEPATITIS E VIRUS (HEV) ANDTHEIR USE IN SERODIAGNOSIS OF HEV INFECTION

摘要: 目的：为诊断戊型肝炎，寻找更好的诊断试剂盒。方法:根据戊型肝炎病毒(HEV)蛋白含有2个可能的抗原表位，从戊型肝炎病毒中国株蛋白的氨基酸序列中选取S30，NS33和S42 3段抗原肽，用固相合成法进行化学合成，所得目标肽段纯化后经质谱和氨基酸组成分析确证。结果：本文试剂盒与国外品进行比较，血清分析结果表明，前者质量与日本产品相当，明显优于美国Genelabs试剂盒。结论：以这3个合成的抗原肽作为包被抗原组装的戊型肝炎病毒血清抗体的ELISA诊断试剂盒有较好的灵敏性和专一性，可用于临床检测和流行病调查。

Abstract: AIM: For the diagnosis of hepatitis E to search for specific and sensibly diagnostic kit. METHODS and RESULTS: Since the protein of hepatitis E virus (HEV) contains two potential immunodominant epitopes., three fragments (S30, S42 and NS33) have been chosen from the amino acid sequence of the protein of the HEV genome of Chinese strain and synthesized by using solid phase method. They were characterized by amino acid analysis and mass spectroscopy. An enzyme-linked immunosorbent assay (ELISA) kit was developed by using these three synthetic peptides. CONCLUSION: This kit show high specificity and sensitivity in serodiagnosis and will be applicable to clinic diagnosis.