Abstract: The micellar thin layer chromatographic behaviours of rutin, quercetin and morin were studied. The methods of separation and identification were established:Polyamide thin coating was used as the stationary phase and 4% Zeph- acetylacetoneH₂O (5:1.5:1.5) used as the mobile phase. The method was applied to the separation and identification of quercetin and rutin in samples of Sophorae flos and satisfactory result was obtained.