毛细管电泳法测定人血浆胸腺九肽的含量

CAPILLARY ZONE ELECTROPHORESIS FOR DETERMINATION OF THYMULIN IN HUMAN PLASMA

摘要: 目的建立定量测定人血浆中胸腺九肽的毛细管区带电泳法。方法运行缓冲液为50 mmol·L^-1 Na₂B₄O₇-10 mmol·L^-1 Na₂HPO₄ (pH 9.10)。毛细管总长57 cm,有效长度50 cm,内径75 μm。运行电压12 kV,运行温度25℃。检测波长:200 nm。结果胸腺九肽的线性范围为1～10 μg·mL^-1,γ=0.9990,重现性RSD为3.05%,回收率为70.97%,RSD为3.32%。结论毛细管电泳法可检测人血浆胸腺九肽的含量,方法简便、可靠、快速。

Abstract: AIMTo establish a capillary zone electrophoresis (CZE) method for determination of thymulin in human blood plasma. Thymulin is a nonapeptide produced by the thymic epithelium. METHODSThe uncoated capillary used was 75 μm ID×57 cm (50 cm effective length). The experimental conditions were as follows: the running buffer was phosphate-borate buffer containing 50 mmol·L^-1 Na₂B₄O₇-10 mmol·L^-1 Na₂HPO₄ (pH 9.10); running voltage was 12 kV; operated temperature was 25℃; running time was 13 minutes, detective wavelength was 200 nm. RESULTSThe linear range of the thymulin standard curve was 1～10 μg·mL^-1, γ=0.9990, the average recovery was 70.97% with RSD of 3.32%, the RSD of reproducibility was 3.05%. CONCLUSIONThe method of CZE for determination of thymulin in human plasma is simple and reliable.