逍遥丸的三维高效液相色谱法鉴定和指标成分的定量

THE IDENTIFICATION AND DETERMINATION OF MARKER SUBSTANCES IN XIAOYAO WAN BY THREE DIMENSIONAL HPLC

摘要: 用三维高效液相色谱(three dimensional HPLC)法对中成药逍遥丸进行了分析。结果表明该法用于逍遥丸中单味中药鉴定及其指标成分(marker substances)含量测定均得到满意结果。

Abstract: A Chinese traditional patent medicine Xiaoyao Wan was analysed by threedimensinal HPLC. The result shows that the Chinese traditional medicines involved in Xiaoyao Wanwere identified excellently and the content of marker substances can be determined accurately. According to the results of determination, the content of saikosaponin b₁, b₂, glycyrrhizin,paeoniflorin and atractylenolide Ⅲ in Xiaoyao Wan was 0. 43, 0. 53, 5. 22, 3. 95 and 0. 16(mg/g), respectively); The RSD was 1.2, 0. 8, 0. 4, 0. 3 and 1.0 (%) respectively.