Abstract: This paper provides the basic principle and experimental technique of differential-linear combination equal value derivative spectrophotometry. Quantitative analysis of three or four component-mixture can be done without separation. This method was investigated to assay the contents of chlorphenamine and propantheline bromide in Pukeling tablets by using UV-210A spectrophotometer. The average recoveries of chlorphenamine and propantheline bromide were 100. 2±1.49% (CV) and 99.89± 1.03% (CV)respectively. The results showed that it not only can effectively remove distrubances from each other, but also give higher sensitivity and accuracy.