Abstract: Three compounds have been isolated from the dichloromethane soluble fraction of the fruiting body of Ganoderma lucidum (Fr.) Karst. On basis of spectral analyses (UV, IR, MS,¹HNMR, ¹³CNMR and 2D-NMR), they were identified as 3,7-dioxo-lanosta-8,24(E)-dien-26-oic acid (I), 7β-15α-dihydroxy-3,11,23-trioxo-5α-lanost-8-en-26-oic acid (II) and 3β,7β,15α-trihydroxy-11,23-dioxo-5α-lanosta-8-en-26-oic acid (III). Compound I is a new compound named ganoderic acid DM.