Abstract: In this paper, the photodynamic effect, photosensitive side action and acute toxicity of three China-made new hematoporphyrin derivatives, HPS, LF-019 and Y-HPD were compared with those of America-made photofrin Ⅱ. The experimental method included: (1) the killing effect of photosensitizer plus exposure to light on MGC-803 cells, (2) the survival time of mice that received L₆₁₅ cells pretreated with photosensitizer and light, (3)the inhibitory effect on ³H-Td R or ³H-UR incorporated into DNA or RNA, (4) the uptake and storage of photosensitizers in L₁₂₁₀ cells, (5) the ear index and skin index that showed skin photosensitive side action and (6) the LD₅₀ in mice.The results showed that the photodynamic effect of the three China-made photosensitizers was more potent than that of photofrin Ⅱ. In the three China-made photosensitizers, except for the skin photosensitive side action, Y-HPD was more potent than HPS or LF-019 on the killing effect of MGC-803 cells, the survival time of L₆₁₅ mice, the inhibitory effect of nucleic acid biosyntnesis and the content of photosensitizers in L₁₂₁₀ cells. The acute toxicity of Y-HPD was lower than that of other photosensitizers.