卡西霉素对血小板在脑微血管内皮细胞粘附的影响及药物的阻断作用

EFFECTS OF CALCIMYCIN ON PLATELET ADHESION TD CULTURED BOVINE CEREBRAL MICROVASCULAR ENDOTHELIAL CELLS AND ITS ANTAGONISM BY DRUGS

摘要: 体外实验表明calcimycin0.01μmol·L^-1刺激牛脑微血管内皮细胞25min,使兔血小板与脑微血管内皮细胞的粘附率增高17.1%。PAF受体拮抗剂WEB20860.1,1.0和10.0μumol·L^-1对血小板在脑微血管内皮细胞上粘附的抑制率分别为9.0,22.9和23.1%。DMPP0.1,1.0,10.0μmol·L^-1及Tet0.1,1.0,10。Oμmol·L^-1的抑制率分别为9.7,15.6,22.1%和7.8,15.6及24.6%,提示DMPP和Tet对脑血管有保护作用。

Abstract: Calcimycin induced rabbit platelet adhesion to cultured bovine cerebral microvascu-lar endothelial cells(CMEC)and its inhibition by triazelodiazepine(WEB 2086),1,5-bis-(3,4-dimethoxyphenyl)-tetrahydro-(4H)-pyran(DMPP)and tetrandrine(Tet )were investigated.TheresuIts showed that calcimycin significantly increased platelet adhesion to CMEC,The plateiet adhesionto CMEC was increased by 17.1%:VS controf after stimulation with calcimycin0.01μmol·L^-1 for25 min, WEB 2086 0.1,1.0and l0.0μmol·L^-1 or DMPP 0.1,1. 0 and l0.0μmol·L^-1or Tet0.1,1. 0 and l0.0μmol·L^-1 inhibited the calcimycin induced platelet adhesion to CMEC by 9.0,22.9 and 23.1%or9.7, 15.6 and 22.1%or 7.8, 15.6 and 2.6%, respectively.This indicatesthat DMPP and Tet may have perspectives in the prevention af1d treatment of cerebral vascular diseases.