The paper reports the systematic study on felodipine and its impurities in tablets, to improve its quality standards for the control of the related substances.  HPLC-DAD, UPLC-MS, IR and NMR methods were used for the isolation of felodipine and its impurities in tablets, their identification and the zebrafish animal model was used for the analysis of the toxic impurities.  In felodipine material and its tablets, three impurities are isolated and identified.  They are impurity 1 dimethyl 4-(2, 3-dichlorophenyl)-2, 6-dimethyl-1, 4-dihydropyridine-3, 5- dicarboxylate, impurity 2 ethyl methyl 4-(2, 3-dichlorophenyl)-2, 6-dimethylpyridine-3, 5-dicarboxylate and impurity 3 diethyl 4-(2, 3-dichlorophenyl)-2, 6-dimethyl-1, 4-dihydropyridine-3, 5-dicarboxylate, separately.  The result of zebrafish animal model analysis showed that the teratogenic effects of four compounds were: impurity 3 ≥ felodipine > impurity 1 > impurity 2, lethal effects were as follows: impurity 2 = impurity 3 > felodipine ≥ impurity 1.  This study confirmed the toxicity of three impurities in felodipine.  According to the results, the paper suggested the amendments to the standard of the medicine and provided the support to the control of impurities in the manufacturing process.