冯华松, 张勇, 汪海. 吡那地尔对低氧低糖诱导神经细胞凋亡的抑制作用J. 药学学报, 2001, 36(11): 812-816.
引用本文: 冯华松, 张勇, 汪海. 吡那地尔对低氧低糖诱导神经细胞凋亡的抑制作用J. 药学学报, 2001, 36(11): 812-816.
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FENG Hua-song, ZHANG Yong, WANG Hai. INHIBITORY EFFECTS OF PINACIDIL ON APOPTOSIS OF PRIMARY CULTURED CORTICAL NEURONS INDUCED BY HYPOXIA-HYPOGLYCEMIAJ. Acta Pharmaceutica Sinica, 2001, 36(11): 812-816.
Citation: FENG Hua-song, ZHANG Yong, WANG Hai. INHIBITORY EFFECTS OF PINACIDIL ON APOPTOSIS OF PRIMARY CULTURED CORTICAL NEURONS INDUCED BY HYPOXIA-HYPOGLYCEMIAJ. Acta Pharmaceutica Sinica, 2001, 36(11): 812-816.
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吡那地尔对低氧低糖诱导神经细胞凋亡的抑制作用

INHIBITORY EFFECTS OF PINACIDIL ON APOPTOSIS OF PRIMARY CULTURED CORTICAL NEURONS INDUCED BY HYPOXIA-HYPOGLYCEMIA

    摘要
  • 摘要: 目的　探讨ATP敏感性钾通道(KATP)开放剂吡那地尔(Pin)对低氧低糖再复氧诱导的大鼠皮质神经细胞凋亡的影响。方法　用电镜和流式细胞分析技术,检测原代培养的大鼠皮质神经细胞凋亡。结果　低氧低糖再复氧可诱导大鼠皮质神经细胞凋亡。Pin (10-5,10-6,10-7mol·L-1 ) 3个剂量组均能降低凋亡细胞百分率,以10-5mol·L-1 组效果最好。上述作用可被KATP特异性阻断剂格列苯脲(Gli)所拮抗。结论　Pin对低氧低糖再复氧诱导的大鼠皮质神经细胞凋亡有明显的抑制作用,提示KATP通道在调控细胞凋亡的过程中可能起着重要的作用。

     

    Abstract: AIM To investigate the effects of ATP-sensitive potassium channel (KATP) opener pinacidil (Pin) on apoptosis of primary cultured cortical neurons induced by hypoxia-hypoglycemia and reoxygenation. METHODS Apoptosis was measured using electron microscope and flow cytometric analysis technique. RESULTS Hypoxia-hypoglycemia and reoxygenation was shown to induce apoptosis of cortical neurons in primary culture. Pin (10-5, 10-6, 10-7mol·L-1) was found to decrease the percent of apoptosis. Pin (10-5mol·L-1) was found to improve ultrastructural features of apoptosis of cortical neurons induced by hypoxia-hypoglycemia and reoxygenation. These effects were reversed by glibenclamide (Gli), the specific antagonist of KATP. CONCLUSION Pin showed inhibitory effects on apoptosis of cortical neurons induced by hypoxia-hypoglycemia and reoxygenation.These results indicate that KATP plays important role in regulating the process of apoptosis.

     

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