苦参碱对细菌脂多糖诱导大鼠枯否细胞释放肿瘤坏死因子及白细胞介素-6的影响

INHIBITORY EFFECT OF MATRINE ON LIPOPOLYSACCHRIDE INDUCED TUMOR NECROSIS FACTOR AND INTERLEUKIN-6 PRODUCTION FROM RAT KUPFFER CELLS

摘要: 目的是研究苦参碱对细菌脂多糖(lipopolysachrides,LPS)诱导经卡西霉素(calcimycin,Cal)预激活的大鼠枯否细胞分泌肿瘤坏死因子(tumor necrosis factor,TNF)、白细胞介素-6(interleukin-6,IL-6)的影响以及对小鼠体内产生TNF和IL-6的影响。结果,苦参碱125,250及500mg·L^-1剂量依赖性抑制大鼠枯否细胞分泌TNF和IL-6;苦参碱50及100mg·kg^-1降低小鼠体内TNF和IL-6的水平。提示苦参碱的抗炎作用可能与其抑制TNF及IL-6的产生有关。

Abstract: The effect of matrine on the lipopolysacchride (LPS)-induced tumor necrosis factor and interleukin-6 production from rat Kupffer cell was investigated. Results showed that matrine 125, 250 and 500 mg·L^-1 suppressed TNF and IL-6 production from Cal primed Kupffer cells in the presence of lipopolysacchrides (LPS, 100 μg·L^-1) in a concentration dependent manner. Treatment with matrine 50 and 100 mg·kg^-1 before LPS injection(3.5 mg·kg^-1) markedly decreased mouse serum TNF and IL-6. The results suggest that matrine may have protective effect on LPS-inuced liver injury.