Abstract: To study the metabolism of trans-resveratrol-3-O-glucoside (TRG) in vitro in rat tissues, the incubation with cell-free extracts from rat stomach, duodenum, jejunum, ileum and liver was performed, separately. After TRG was incubated with the tissue extracts at 37 ℃ for up to 90 min, the deglycosylation of TRG was (3.50±0.24)% for stomach, (65.7±5.94)% for duodenum, (83.5±6.43)% for jejunum, (77.6±6.26)% for ileum and (9.62±1.21)% for liver, separately. It was observed that the small intestine extracts were more active in deglycosylation of TRG than the liver extract, which suggested that the small intestine mucosa played an important role in deglycosylation of TRG. It was assumed that the deglycosylation of TRG was catalyzed by β-glucosidase in small intestine mucosa.