固相萃取结合GC-FID和GC-MS快速分析血浆中巴比妥类药物

RAPID IDENTIFICATION AND QUANTITATION OF BARBITURATES IN PLASMA USING SOLID-PHASE EXTRACTION COMBINED WITH GC-FID AND GC-MS METHOD

摘要: 报道了用X-5树脂固相萃取分离纯化、GC-FID和GC-MS定量和定性分析血浆中巴比妥类药物的方法,含药物的血浆可直接用萃柱处理,操作简单方便,用甲醇-乙酸乙酯作为洗脱剂,不同药物的相对回收率为95～105.4%,RSD为3.46～6.41%。检测灵敏度及线性范围适合血药浓度的监测及中毒急救分析的需要。

Abstract: A rapid screening method for the quantitation and identification of 7 barbituratesin plasma is presented.The method employs solid- phase extraction at pH 3 using X-5 resin asadsorbent.Identification of each barbiturate is achieved by retention time and GC- MS. Detectionthreshold(0.3～0.5ug·ml^-1 in a 0. 5 ml plasma sample and l ul injection volume),precision(RSD 3.46～6. 41%),and linearity(r>0.99)were satisfactory for blood level monitoring andclinical toxicological applications.