人尿中右美沙芬及代谢物的高效液相色谱测定法

HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF DEXTROMETHORPHAN AND ITS METABOLITE IN HUMAN URINE

摘要: 用反相高效液相色谱法测定人尿中右美沙芬(DM)及其代谢物去甲右美沙芬。尿样经酸、碱提取纯化,直接进样HPLC系统。样品经苯基柱分离后在荧光(激发波长280nm,发射波长310nm)下检测,以乙腈—含10mmol·L^-1 KH₂PO₄,10mmol·L^-1庚磺酸盐水溶液(35∶65,pH4.0)为流动相,外标法定量。DM的最低检测浓度为0.023mg·L^-1。DM和DX的日内和日间相对标准差均小于5%。平均回收率为104.90%。本法为临床筛选异喹胍羟化酶活性与合理用药提供了一种简便、安全的方法。

Abstract: A reverse-phase high-performance liquid chromatographic method was established for the determination of dextromethorphan (DM) and its metabolite dextrophan (DX) in human urine. The sample was purified by an acid-base extraction followed by HPLC analysis on a phenyl column. The eluate was monitored by fluorescence at 280 nm (excitation) and 310 nm (emission). The limit of detection of DM was 0.023 mg·L^-1. The within-day and between-day RSD% of DM and DX were all lower than 5%. The average recovery was 104.78%.