To investigate the chemical constituents of Chamaecyparis obtusa var. breviramea f. crippsii, various column chromatography and spectroscopic methods were used for the isolation and elucidation of compounds.  One new monoterpenoid glucoside, (4S)-4-isopropylcyclohex-1-enecarboxylic acid 4-O- β-D-glucopyranoside (1), together with five known compounds, (4R)-p-menth-1-ene-7, 8-diol 7-O-β-D-glucopyranoside (2), skimmin (3), 7-6-O-(6-deoxy-α-L-mannopyranosyl)-β-D-glucopyranosyloxy-2H-1-benzopyran-2-one (4), stigmast-4-en-3-one (5) and 1, 4-benzenedicarboxylic acid 1-butyl-4-(2-methylpropyl)  ester (6) were isolated and identified from the twigs of this plant.  All compounds were isolated from this plant for the first time.  The methanol extract of this plant showed cytotoxicity on cancer cell lines A549, BGC-823, Du145 and MDA-MB-231 with IC₅₀ values of 0.94, 1.07, 0.95 and 0.96 μg·mL⁻¹, respectively.  Yet, compounds 1, 2 and 3 showed no cytotoxicity on cancer cell lines HeLa, BGC-823 and A549.