高效毛细管电泳测定血管紧张素转化酶抑制剂captopril的活性

Determination of the inhibitory activity of angiotensin-converting enzyme inhibitor captopril by high performance capillary electrophoresis

摘要: 目的建立高效毛细管电泳测定血管紧张素转化酶抑制剂captopril抑制活性的方法。方法用紫外分光光度计确定了马尿酸的特征吸收波长为228 nm。高效毛细管电泳的条件：熔融石英毛细柱，电泳缓冲液为pH 8.3的50 mmol·L^-1磷酸缓冲液，进样压力4.8 kPa，进样时间3 s，分离电压20 kV，检测波长228 nm。结果在7 min内使反应物和生成物完全分离，标定了captopril的IC₅₀值为0.019　μmol·L^-1，经过酶反应动力学判断captopril为竞争性抑制剂。结论该方法准确、简便、快速，可用于captopril抑制活性的分析。

Abstract: AimTo establish a method for determinate of the inhibitory activity of angiotensin-converting enzyme inhibitor captopril by high performance capillary electrophoresis. MethodsThe characterstic absorptive wavelength of hippuric acid determined by ultraviolet spectrophotometer is 228 nm. The method employed a melted capillary column, 50 mmol·L^-1 phosphoric acid (pH 8.3) buffer solution, inject pressure 4.8 kPa, inject time 3 s, separation voltage 20 kV and detection wavelength 228 nm. ResultsThe reactant and resultant was separated completed within 7 min. IC₅₀ of captopril was 0.019 μmol·L^-1. Captopril is a competitive inhibitor, which was proved by enzyme reaction dynamics. ConclusionThe method was shown to be accurate, simple and rapid and can be used for determination of the inhibitory activity of captopril.