基于环氧合酶抑制作用的人工麝香质量评价方法研究

Quality evaluation of artificial musk based on its inhibitory effect on cyclooxygenase-2

摘要:
采用酶免疫 (EIA) 法, 测定和计算人工麝香对环氧合酶Ⅱ (COX-2) 的抑制率, 构建人工麝香对COX-2抑制作用的量效关系, 为建立人工麝香的体外抗炎效价测定方法提供研究基础。结果显示, 人工麝香对COX-2具有明显抑制作用, 半数抑制浓度 (IC₅₀) 为2.26 mg·mL⁻¹, 在0.31～20.0 mg·mL⁻¹内, 具有明显的量效关系。结果表明, 该方法快速、灵敏、准确、重现性好, 可用于建立人工麝香抗炎生物效价检测方法, 同时本文也为人工麝香的生物活性评价提供了新的研究思路和方法。

Abstract:
The inhibitory ratio (I %) of artificial musk on cyclooxygenase-2 (COX-2) was determined by enzyme immunoassay (EIA). The dose-effect relationship between concentrations of artificial musk and I % was established. It was found that artificial musk had obvious inhibitory action on COX-2. The concentration for 50% of maximum inhibitory effect (IC₅₀) was about 2.26 mg·mL⁻¹. There was a good relationship between the logarithm concentrations of artificial musk and I % when the concentrations of artificial musk ranged from 0.31−20.0 mg·mL⁻¹. The results indicated that this EIA method could be applied to evaluate the anti-inflammatory activity of artificial musk quickly, conveniently, sensitively and exactly. This paper provided a novel method and foundational research for the bioassay of artificial musk.