唐古特山莨菪毛状根对去氢表雄酮的生物转化

Biotransformation of dehydroepiandrosterone by hairy root cultures of Anisodus tanguticus

摘要: 目的通过生物转化对去氢表雄酮进行结构修饰，以期获得更有意义的转化产物。方法利用唐古特山莨菪毛状根液体悬浮培养体系对去氢表雄酮进行生物转化，通过柱色谱及制备薄层色谱进行分离纯化，利用核磁共振、ESI-MS等光谱鉴定结构。结果分离得到了5个转化产物，即：androst-4-ene-3,17-dione ( I)； 6α-hydroxyandrost-4-ene-3, 17-dione (II)；6α,17β-dihydroxyandrost-4-ene-3-one(III)； androst-4-ene-3,6,17-trione (IV)；17β-hydroxyandrost-4-ene-3-one (V)。结论所得到的5个化合物均为首次通过植物组织培养生物转化的方法从唐古特山莨菪毛状根液体培养体系中分离得到。

Abstract: AimTo modify the structure of dehydroepiandrosterone (DHEA) . MethodsUsing hairy root cultures of Anisodus tanguticus to perform biotransformation of DHEA, using chromatographic and spectral techniques to isolate and identify the products. Results(1) The MS medium without plant hormone was suitable for the growth of the hairy root. (2) DHEA was converted into five products: androst-4-ene-3,17-dione (I)； 6α-hydroxyandrost-4-ene-3 ,17-dione (II) ； 6α,17β-dihydroxyandrost-4-ene-3-one (III)； androst-4-ene-3,6,17-trione (IV) and 17β-hydroxyandrost-4-ene-3-one (V). Conclusion It is the first time to use hairy root cultures of Anisodus tanguticus for the biotransformation of DHEA and five DHEA-related compounds were obtained.