Abstract: Based on optimizing the formulations of artificial reconstituted pulmonary surfactant (APS) used for the prevention and treatment of adult respiratory distress syndrome (ARDS) by a surface chemical method, a quartz lung animal model to obtain active alveolar macrophages (AMs) was established, using a bioluminesence technique to optimize APS’s formulation according to the free-radical-scavenging abilities of APS. An optimal formulation was obtained, which showed good surface properties and free radical scavenging abilities. The APS preparation will be used in ARDS animal model test.