基于HMGR、SQS1、β-AS基因CNVs的甘草道地性机制研究
Mechanism of genuineness of liquorice Glycyrrhiza uralensis based on CNVs of HMGR, SQS1 and β-AS gene
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摘要:
本文利用real-time PCR方法对不同产地甘草的3-羟基-3-甲基戊二酰CoA还原酶 (3-hydroxy-3-methylglutaryl-CoA reductase, HMGR)、鲨稀合成酶1 (squalene synthetase 1, SQS1)、β-香树脂醇合成酶 (β-amyrin synthase, β-AS) 基因的拷贝数进行了研究, 发现不同产地的HMGR基因存在1～3个拷贝数变异, SQS1基因存在1～2个拷贝数变异, 未发现β-AS基因拷贝数变异。甘草HMGR、SQS1、β-AS基因拷贝数存在5种自然组合类型: A型 (2+1+1)、B型 (1+1+1)、C型 (3+2+1)、D型 (2+2+1) 和E型 (3+1+1), 其中内蒙古杭锦旗甘草存在A、B两种类型, A与B的比例为1∶1.3; 内蒙古赤峰甘草也存在A、B两种类型, 但A与B比例为3∶1; 宁夏盐池甘草存在A、B、C、D 4种类型, A/B/C/D比例为1∶5.1∶1∶2, A/B比例为1∶5.1; 甘肃民勤甘草存在A、B、E 3种类型, A/B/E比例为4.1∶2.1∶1, A/B比例为2∶1。本研究证明中药功能基因基因组拷贝数变异 (copy number variations, CNVs) 与产地具有相关性, 可能是道地药材形成的机制之一。
Abstract:This study is to reveal the correlation between CNVs of HMGR, SQS1, β-AS gene and genuineness of liquorice. Real-time PCR was used to detect the copy number of HMGR, SQS1, β-AS gene of liquorice. According to the results, the range of the copy number variation of HMGR gene was between 1 and 3, the copy number of SQS1 gene was 1 or 2, and the copy number of β-AS gene was only 1. On the basis of the copy number of HMGR, SQS1 and β-AS gene, there were five groups, type A (2+1+1), type B (1+1+1), type C (3+2+1), type D (2+2+1) and type E (3+1+1). There were two types, type A and type B, in Hangjinqi of Inner Mongolia, and the ratio of A to B was 1∶1.3. There were also two types, type A and type B, in Chifeng of Inner Mongolia, and the ratio of A to B was 3∶1. There were four types, type A, type B, type C and type D, in Yanchi of Ningxia province, and the ratio of A to B was 1∶5.1. There were three types, type A, type B and type E, in Minqin of Gansu province, and the ratio of A to B was 2∶1. So CNVs mainly existed in the liquorice from Ningxia and
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