抗分枝杆菌活性成分——丹参酮的胆汁排泄与肝内转化

TANSHINONES: ANTIMYCOBACTERIAL AGENTS——ITS BILE EXCRETION AND BIOTRANSFORMATION IN RAT LIVER

摘要: 大鼠由十二指肠给丹参酮Ⅰ、丹参酮Ⅱ_A、隐丹参酮及丹参的乙醇粗提物(总酮)后,药物可由胆汁排出。在胆汁中的含量采用高效液相色谱法测定。实验发现给总酮组胆汁中丹参酮Ⅱ_A的排出量为丹参酮Ⅱ_A组排出量的5～10倍。在给隐丹参酮组,发现隐丹参酮在肝内可能转化为丹参酮Ⅱ_A用抑菌指标亦可在肝内检出抗分枝杆菌607的活性成分。

Abstract: Tanshinone Ⅰ, Tanshinone Ⅱ_A, Cryptotanshinone, total Tanshinones and alcoholic extract of Dan-Shen, the root of Salvia miltiorrhiza Bange, were given intraduodenally in rats. The concentration of Tanshinones excreted in bile were determined by HPLC. It was found that the concentration of Tanshinone Ⅱ_A excreted in bile in the group of alcoholic extract of Dan-Shen was five to ten times as much as in the group of Tanshinone Ⅱ_A. It was also shown that Cryptotanshinone can be biotransformed into Tanshinone Ⅱ_A by dehydrogenation in the liver. The active substances against mycobacterium-607 in liver can also be detected by microbiological assay.