Abstract: The result of permeability test of ketoprofen(KP) in vitro showed that the penetra-tion process could be described by zero-order kinetics,Azone(AZ),oleic acid(OA),1-methyl 2-pyrrolidone(2P),dimethylformamide(DMF),propylene glycol(PG)and lauryl alcohol(LA),sixpenetration enhancers,on the percutaneous absorption of KP were carried out by using isolated shavedmouse skin,These enhancers exhibited different effects,Among the enhancers,OA,AZ,2P and PGenhanced the skin permeation of KP,Their order of effect was OA>AZ>2P>PG,AZ and 2P incombination with OA were shown to have synergistic effect.But DMF,LA and PG at high levelsinhibited the permeation of KP.