Abstract: AIM: The neuroprotective effect of KNL(components:L-lysine monohydrochloride∶Vit B1∶Vit B6=900∶3.5∶2) against acute iterative anoxia was studied with quantitative analysis of electrocorticogram(ECoG). METHODS: Quantitative analysis method was used to analyze the duration of disappearance of ECoG and its recovery, and, in turn, to appraise the neuroprotective effect of KNL on anoxia. RESULTS: On the acute iterative anoxia model of rats, KNL(0.63, 1.26 g.kg^-1 ig) was shown to markedly and dose-dependently prolong the time of ECoG′s average amplitude reaching the lowest level or the isoelectric point in the 2-min anoxia group and promote the recovery of ECoG in the 3-min anoxia group. The difference was significant compared with the normal saline group(P<0.01) and insignificant compared with the flunarizine-pretreated (2.5 mg.kg^-1 ig) group(P>0.05). In the 2-min anoxia group pretreated with 1.26 g.kg^-1 ig, ECoG did not reach the isoelectric point and the lowest average amplitude was 10 percent that of nomoxia. KNL at different doses decreased the average amplitude of ECoG in different doses groups.CONCLUSION: KNL ig in certain range of doses has neuroprotective effect on reiterate anoxia in rats.