女儿茶多糖NLC-A的分离纯化及化学结构

STUDIES ON ISOLATION, PURIFICATION AND STRUCTURE OF NLC-A OF RHAMNUS HETEROPHYLLUS

摘要: 女儿茶为民间常用中草药。经水提取,去蛋白及小分子杂质得到女儿茶多糖(NLC-A)粗制品。再经Cellex D,Sepharose 6B和Sephadex G-100相继柱层析,得到均一组分NLC-A,分子量为1.2×10⁴,NLC-A经全水解,甲基化反应,过碘酸盐氧化,Smith降解,KI-I₂反应和IR证明其结构为以α(1→4)-D-葡萄糖为主链,并在o-6位有分枝的葡聚糖。

Abstract: A water-soluble gluean, NLC-A, from the roots of Rhamnus heterophyllus Olive was obtained as a homogeneous component as determined by HPLC. NLC-A was shown to compose of D-glucose, and its MW was estimated to be 1.2×10~4.Methylation analysis, peroxidation analysis, Smith degradation and reaction with I₂-KI showed that NLC-A is a (1→4)linked α-D-glucan to which are attached glucosyl side chains at o-6 of the glucosyl residues of the main chain.