张婷, 徐珞珊, 王峥涛, 周开亚, 张宁, 史永峰. 药用植物束花石斛、流苏石斛及其形态相似种的PCR-RFLP鉴别研究药用植物束花石斛、流苏石斛及其形态相似种的PCR-RFLP鉴别研究J. 药学学报, 2005, 40(8): 728-733.
引用本文: 张婷, 徐珞珊, 王峥涛, 周开亚, 张宁, 史永峰. 药用植物束花石斛、流苏石斛及其形态相似种的PCR-RFLP鉴别研究药用植物束花石斛、流苏石斛及其形态相似种的PCR-RFLP鉴别研究J. 药学学报, 2005, 40(8): 728-733.
shu
ZHANG Ting, XU Luo-shan, WANG Zheng-tao, ZHOU Kai-ya, ZHANG Ning, SHI Yong-feng. Molecular identification of medicinal plants: Dendrobium chrysanthum, Dendrobium fimbriatum and their morphologically allied species by PCR-RFLP analysesJ. Acta Pharmaceutica Sinica, 2005, 40(8): 728-733.
Citation: ZHANG Ting, XU Luo-shan, WANG Zheng-tao, ZHOU Kai-ya, ZHANG Ning, SHI Yong-feng. Molecular identification of medicinal plants: Dendrobium chrysanthum, Dendrobium fimbriatum and their morphologically allied species by PCR-RFLP analysesJ. Acta Pharmaceutica Sinica, 2005, 40(8): 728-733.
shu

药用植物束花石斛、流苏石斛及其形态相似种的PCR-RFLP鉴别研究药用植物束花石斛、流苏石斛及其形态相似种的PCR-RFLP鉴别研究

Molecular identification of medicinal plants: Dendrobium chrysanthum, Dendrobium fimbriatum and their morphologically allied species by PCR-RFLP analyses

    摘要
  • 摘要: 目的建立简易的采用rDNA ITS区作为分子标记对中药石斛的基源植物进行分子鉴定的技术。方法 用聚合酶链反应—限制性片段长度多态性(PCR-RFLP)方法获得ITS片段的限制性图谱。束花石斛及其形态相似种的PCR扩增产物用Cla I和Apa LI酶切，流苏石斛及其形态相似种的PCR扩增产物用Sph I酶切。结果根据预测的PCR-RFLP图谱，可以鉴别药用植物束花石斛、流苏石斛及它们的形态相似种。用这种方法鉴定了市场上收集的25件商品束花石斛、流苏石斛新鲜药材的原植物。结论rDNA ITS的PCR-RFLP可用于鉴定石斛药材的原植物，具有简便、费用低的优点。

     

    Abstract: AimTo establish a simple method for molecular identification of original plants of D.chrysanthum and D.fimbriatum using molecular marker rDNA ITS region. MethodsRestriction patterns of ITS fragments were obtained using PCR-RFLP method. The PCR products of D.chrysanthum and its morphologically allied species were digested at 37 ℃ by Cla I and Apa LI, those of D.fimbriatum and its morphologically allied species were digested by Sph I. ResultsD.chrysanthum, D.fimbriatum and their morphologically allied species could be identified by predicted restriction profiles of PCR-RFLP. The botanical origin of twenty-five fresh samples of “Shihu” collected in markets was identified by this method. ConclusionThe results showed that PCR-RFLP analysis of the rDNA ITS region is a feasible, simple and inexpensive method for determining the botanical origin of the traditional Chinese medicine “Shihu”.

     

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