侯进, 弥曼, 魏明, 易文辉. 金属特性碳纳米管的筛选分离及对人类乳腺癌细胞的光热效应J. 药学学报, 2015,50(8): 980-985.
引用本文: 侯进, 弥曼, 魏明, 易文辉. 金属特性碳纳米管的筛选分离及对人类乳腺癌细胞的光热效应J. 药学学报, 2015,50(8): 980-985.
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HOU Jin, MI Man, WEI Ming, YI Wen-hui. Photothermal effects of metallic carbon nanotubes on human breast cancer cellsJ. Acta Pharmaceutica Sinica, 2015,50(8): 980-985.
Citation: HOU Jin, MI Man, WEI Ming, YI Wen-hui. Photothermal effects of metallic carbon nanotubes on human breast cancer cellsJ. Acta Pharmaceutica Sinica, 2015,50(8): 980-985.
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金属特性碳纳米管的筛选分离及对人类乳腺癌细胞的光热效应

Photothermal effects of metallic carbon nanotubes on human breast cancer cells

    摘要
  • 摘要: 分离并研究金属碳纳米管 (m-SWNTs) 的光热效应及其对乳腺癌细胞的抑制作用。采用PmPV (poly (m-phenylenevinylene)-alt-(p-phenylenevinylene)) 从单壁碳纳米管 (SWNTs) 中分离得到具有金属特性碳纳米管, 测定其光热效应, 与MCF-7乳腺癌细胞共同培养, 在808 nm波长下, 用红外光 (NIR) 以2 W·cm-2的强度照射细胞3 min, MTT比色法测定m-SWNTs对MCF-7细胞的杀灭作用; 流式细胞术测定m-SWNTs对MCF-7细胞凋亡的影响。结果表明, 用PmPV可有效分离m-SWNTs, 其光热效应明显增强; 可以抑制MCF-7细胞的存活率, 促进MCF-7细胞凋亡, 其效果强于未经分离的SWNTs。通过PmPV分离的m-SWNTs具有更高的光热转化效率, 并且对MCF-7细胞具有更强的杀灭效果。

     

    Abstract: SWNTs are a mixture of 1/3 metallic SWNTs (m-SWNTs) and 2/3 semiconducting SWNTs (s-SWNTs). It is desirable to separate the metallic SWNTs from the semi-conducting ones. In this study m-SWNTs was separated by using a poly(m-phenylenevinylene)-alt-(p-phenylenevinylene) (PmPV) derivative and used as photo-thermal media instead of SWNTs. The separation effects of m-SWNTs were evaluated by Raman spectra, molecular modeling and TEM images. The effects of m-SWNTs on MCF-7 cell proliferation and apoptosis were evaluated with MTT assay and flow cytometry, respectively. m-SWNTs were separated with high purity. A strong inhibition of MCF-7 cell growth was observed with the m-SWNTs under near-infrared (NIR) light irradiation. Our results will be helpful for the potential applications of m-SWNTs in clinical photo- thermal cancer therapy.

     

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